Molecular Biology Resources for Hight School Students


	High School Students High School Teachers High School Teachers in Training


	For High School Teachers

	Below are links to lesson plans, PowerPoint lectures, laboratory exercises and background information on molecular biology and molecular genetics. The lesson plans provide an outline of activities for each class and include particular points to emphasize. The PowerPoint lectures are ready for your use in class. Lab exercises/modules provide a list of materials needed and give step-by-step instructions for …and are annotated for teachers … The background information will help you bone up on.

	From DNA to Organism: A Study in DNA Function for the High School Biology Classroom

	• Lab manual Introduction Why create this learning program? How is the program set up? Tips for teaching with your biology textbook Sample timeline for learning program Premodule questionnaire Postmodule questionnaire Lab write-up format Lab write-up scoring checklist Lab write-up scoring sheet premodule: Scientific method and plant growth Explanation Lab letter Teacher suggestions for Premodule Module 1: Phenotypes Explanation Lab letter Module 1 data sheet Teacher suggestions for Module 1 Module 2: DNA and genes Explanation Lab letter Alternate lab introduction: the genetics of the problem Procedures for examining plants’ DNA for the mutation of the AKT-1 gene Part I. Getting out the goods Alternative procedures for examining plants’ DNA for the mutation of the AKT-1 gene Part I. Getting out the goods Part II. Making copies of the suspect Additional materials: PowerPoint: Module2a Part III. Who has the gene? Making the gel Loading your sample into the gel Staining your gel Interpreting your results Additional materials: PowerPoint: Module2b Teacher suggestions for Module 2: DNA extraction (Qiagen DNeasy Plant Mini-Kit) Teacher suggestions for Module 2: DNA extraction (Cartagen Rapid Homogenization Kit: Plant Leaf DNA Amplification) Teacher suggestions for Module 2: PCR A quick primer on how to set up a PCR profile Teacher suggestions for Module 2: Gel electrophoresis Teacher suggestions for Module 2: DNA staining Module 3: Manipulating genes Explanation Lab letter Module 4: Yeast and the gene Explanation Lab letter Quantitative analysis Tips for culturing yeast cells Assessing your cultures after they have grown Qualitative analysis Tips for culturing yeast cells Assessing your cultures after they have grown Teacher suggestions for Module 4: Quantitative analysis Teacher suggestions for Module 4: Qualitative analysis Media prep for yeast culturing Module 5: Structure and function in proteins Explanation Lab letter Teacher suggestions for Module 5: Protein folding lab Making a flapping bird Additional materials: PowerPoint: Module5 Module 6: Genes’ effects on the organism Lab letter Additional materials: PowerPoint: Module6a PowerPoint: Module6b Tips for growing Arabidopsis Growing Arabidopsis in soil Care for the plants Growing Arabidopsis in agar Seed collection Additional information on growing Arabidopsis Growth of plants Growth of plants in sterile conditions Growth of plants on soil Planting on soil Growth conditions Water and nutrition Light Temperature Materials and vendors Premodule Module1 Module2 Module3 Module4 Module5 Module6 Appendix : Some information regarding protein modeling software Works consulted

Contact info:

Dr. Gerald Berkowitz
Agricultural Biotechnology Laboratory, U-4163
Room 302C
1390 Storrs Rd.
University of Connecticut
Storr, CT 06269-4163
E-mail: gerald.berkowitz@
uconn.edu
phone: 860-486-1945

Development of the materials on this web page was supported by grants from: USDA Challenge Education Grants, Secondary and Two-Year
Postsecondary Agricultural Program, Award 01757, Principal Investigator: Jon Swanson, Co-Principal Investigator: Gerald BerkowitzNational
Science Foundation, Division of Molecular Biosciences, Signal Transduction and Cellular Regulation Program, Award 0344141, Principal Investigator: Gerald Berkowitz